[Development of methods for detection of H5N1 from human clinical specimens].

  • 1 June 2006
    • journal article
    • abstracts
    • Vol. 20  (2) , 24-6
Abstract
To establish a method for H5N1 RNA detection and laboratory diagnosis of suspected human avian influenza (H5N1) virus infected cases. The typing and sub-typing primers were designed according to M and H5 and N1 gene respectively, and the RT-PCR and real-time PCR were developed using these primers. The RT-PCR and real-time PCR could be used for H5N1 detection specifically, and there was no cross reaction with other influenza subtypes such as H1 and H3. The sensitivity for RT-PCR and real-time PCR was 1 TCID50 and 0.01 TCID50 respectively. Thirteen laboratory confirmed human H5N1 cases were detected from 42 suspected cases by using these methods. The established RT-PCR and real-time PCR methods can be used for laboratory detection of suspected human H5N1 cases.

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