Studies on ox brain aminopeptides
- 1 November 1967
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 105 (2) , 641-646
- https://doi.org/10.1042/bj1050641
Abstract
Mn2+-inhibited and Mn2+-activated aminopeptidases were observed in ox brain and separated from one another by DEAE- cellulose column chromatography. The Mn2+-inhibited enzyme was purified 36-fold: it exhibits a specificity forfripeptide substrates, whereas the Mn2+-activated aminopeptidase cleaves dipeptldes as well as tripeptldes. Ammonium sulphate treatment generates a Mn2+-stimulated aminopeptidase that is stable to dialysis against EDTA and water, in contrast with an endogenous Mn2+-activated preparation that is irreversibly denatured by such dialysis against EDTA and water.This publication has 22 references indexed in Scilit:
- Serum aminopeptidases, “angiotensinase,” and hypertension—IBiochemical Pharmacology, 1965
- Arylamidase activity related to angiotensinaseBiochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation, 1965
- STUDIES ON DIPEPTIDASES AND AMINOPEPTIDASES .1. DISTINCTION BETWEEN LEUCINE AMINOPEPTIDASE AND ENZYMES THAT HYDROLYZE L-LEUCYL-BETA-NAPHTHYLAMIDE1963
- THE DISTRIBUTION AND ACTIVITY OF CALF BRAIN PEPTIDASES*Journal of Neurochemistry, 1963
- PROPERTIES AND CLASSIFICATION OF SOME BRAIN PEPTIDASES*Journal of Neurochemistry, 1962
- The hydrolysis of dipeptides by different regions of rat small intestineBiochemical Journal, 1960
- Dipeptidase Activity of BrainNature, 1960
- SERUM PEPTIDASES IN HEPATIC DISEASEAnnals of the New York Academy of Sciences, 1958
- LEUCINE AMINOPEPTIDASE .5. ACTIVATION, SPECIFICITY, AND MECHANISM OF ACTION1955
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951