Demequina salsinemoris sp. nov., isolated on agar media supplemented with ascorbic acid or rutin

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Abstract
Three strains, KV-810T, KV-811 and KV-816, were isolated from mangrove soil from a southern island in Japan on media supplemented with ascorbic acid or rutin. These strains contained l-ornithine as the diagnostic diamino acid in the cell-wall peptidoglycan and DMK-9(H4) as the predominant menaquinone. The G+C content of the DNA was 70–72 mol%. These characteristics in combination with 16S rRNA gene sequence analysis revealed that the novel strains belonged to the genus Demequina. The DNA–DNA hybridization values showed that the three new strains belonged to the same species, a novel species of the genus Demequina. Therefore strains KV-810T, KV-811 and KV-816 are proposed as representing a novel species, Demequina salsinemoris sp. nov. The type strain is KV-810T (=DSM 22060T=NBRC 105323T).

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