Genetic Markers on the HTLV-1 p12IProtein Sequences from Brazilian HAM/TSP Patients and Asymptomatic HTLV-1 Carrier Isolates

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Abstract
The human T cell leukemia/lymphotropic virus type-1 (HTLV-1) genome has approximately 9 kb and contains the pX region that codes for regulatory and accessory proteins. The pX ORF-I encodes for the p12 protein, a 99 aa peptide, which presents several functional putative domains, such as leucine zipper motifs, SH3- binding motifs, and a dileucine motif, p12I. Also, a rare p12IK88 allele was found mainly in HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients, suggesting it is a marker of pathogenesis, although recent studies showed p12IK in asymptomatic HTLV-1 carriers. To extend the observations on p12I motifs, we sequenced 26 p12I from HAM/TSP patients and asymptomatic HTLV-1 carriers. Amino acid analysis of 48 p12I motifs demonstrated the presence of several alleles, but the allelic variation, including p12IK, was not prevalent in HAM/TSP isolates. Nonetheless, some genetic markers were recognized in association with isolates from HTLV-1a subgroup B and Brazilian HTLV-1aA strains.