Cadmium- or Zinc-Binding to Bone Lysyl Oxidase and Copper Replacement
- 1 January 1985
- journal article
- Published by Taylor & Francis in Connective Tissue Research
- Vol. 14 (2) , 129-139
- https://doi.org/10.3109/03008208509015019
Abstract
Lysyl oxidase was purified to about 3,000- to 5,000-fold from epiphyseal cartilages of chick embryos. Purification was accomplished by sequential column chromatographies of collagen-sepharose, DEAE-Cellulose and Sephacryl S-200. The molecular weight of the most purified enzyme from 4 different isomers as determined by gel-filtration technique using Sephacryl S-200 column was 32,000 and the purified enzyme contained one copper atom per mole.Keywords
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