Continuous-flow determination of dialyzable calcium in serum.
- 1 July 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 23 (7) , 1258-1263
- https://doi.org/10.1093/clinchem/23.7.1258
Abstract
We describe a procedure for measuring dialyzable calcium in 60 sera per hour with use of AutoAnalyzer (Technicon) modules. The serum is buffered, then dialyzed in a regular 6-inch (15-cm) dialysis block. A constant proportion of the dialyzable calcium is fluorometrically measured. Analysis of samples containing protein, prepared by an equilibrium dialysis technique, shows that protein-bound calcium is not detected. The method requires less than 0.4 ml of serum, and is accurate, precise, and durable. No unusual care or special techniques are required during sample collection or analysis.This publication has 6 references indexed in Scilit:
- Separation and quantitation of serum constituents associated with calcium by gel filtration.Clinical Chemistry, 1976
- The influence of the parathyroid glands on the hypercalcemia of experimental magnesium depletion in the ratJournal of Clinical Investigation, 1968
- An improved automated procedure for the determination of calcium in biological specimensAnalytical Biochemistry, 1967
- ION ASSOCIATION. VI. INTERACTIONS BETWEEN CALCIUM, MAGNESIUM, INORGANIC PHOSPHATE, CITRATE AND PROTEIN IN NORMAL HUMAN PLASMA*Journal of Clinical Investigation, 1961
- Ultracentrifugal Analysis of Protein-bound and Free Calcium in Human SerumJournal of Biological Chemistry, 1960
- THE ULTRAFILTRABLE CALCIUM OF HUMAN SERUM. I. ULTRAFILTRATION METHODS AND NORMAL VALUES 1Journal of Clinical Investigation, 1957