Inducible Promoter-Repressor System from the Lactobacillus casei Phage φFSW
Open Access
- 1 August 2002
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 68 (8) , 4132-4135
- https://doi.org/10.1128/aem.68.8.4132-4135.2002
Abstract
With the aim to extend the presently available inducible gene expression systems for lactobacilli, we have isolated a thermoinducible promoter-repressor cassette from the temperate Lactobacillus casei phage φFSW-TI in Escherichia coli. The φFSW-TI promoter fragment was abutted to the plasmid-borne promoterless β-glucuronidase (gusA) reporter gene and shown to direct its transcription in L. casei. In addition, the functionality of the promoter-repressor system was verified in the L. casei φFSW-TI lysogen by showing that the gusA reporter gene, controlled by the isolated φFSW-TI promoter, was repressed at 28°C and expressed at 42°C. Moreover, a homology search revealed that the C terminus of the isolated φFSW repressor shows a high similarity to the small mutS-related domain of the MutS2 protein family that is unprecedented for phage-encoded repressor proteins.Keywords
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