Identification and Expression of Benzylsuccinate Synthase Genes in a Toluene-Degrading Methanogenic Consortium
- 15 February 2007
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 73 (4) , 1367-1369
- https://doi.org/10.1128/aem.01904-06
Abstract
Benzylsuccinate synthase (BSS) initiates anaerobic toluene biodegradation, and BSS genes have been found in several nitrate- and iron-reducing organisms. Here, two new putative bssA genes were identified in a methanogenic toluene-degrading culture. Transcription was upregulated with toluene but not with benzoate, consistent with the proposed function. These are the first bss sequences from a methanogenic culture.Keywords
This publication has 18 references indexed in Scilit:
- Multiple Reductive-Dehalogenase-Homologous Genes Are Simultaneously Transcribed during Dechlorination by Dehalococcoides- Containing CulturesApplied and Environmental Microbiology, 2005
- New glycyl radical enzymes catalysing key metabolic steps in anaerobic bacteriaBiological Chemistry, 2005
- Genes involved in the anaerobic degradation of toluene in a denitrifying bacterium, strain EbN1Archiv für Mikrobiologie, 2004
- A Real-Time Polymerase Chain Reaction Method for Monitoring Anaerobic, Hydrocarbon-Degrading Bacteria Based on a Catabolic GeneEnvironmental Science & Technology, 2002
- Operon structure and expression of the genes for benzylsuccinate synthase in Thauera aromatica strain K172Archiv für Mikrobiologie, 2001
- Benzylsuccinate Synthase of Azoarcus sp. Strain T: Cloning, Sequencing, Transcriptional Organization, and Its Role in Anaerobic Toluene and m -Xylene MineralizationJournal of Bacteriology, 2001
- Initial reactions of anaerobic metabolism of alkylbenzenes in denitrifying and sulfate-reducing bacteriaArchiv für Mikrobiologie, 1998
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- Evidence That Anaerobic Oxidation of Toluene in the Denitrifying Bacterium Thauera aromatica is Initiated by Formation of Benzylsuccinate from Toluene and FumarateEuropean Journal of Biochemistry, 1996
- GENMARK: Parallel gene recognition for both DNA strandsComputers & Chemistry, 1993