SOFT AGAR CLONOGENIC-ASSAY FOR PRIMARY HUMAN RENAL-CARCINOMA - INVITRO CHEMOTHERAPEUTIC DRUG SENSITIVITY TESTING

  • 1 January 1981
    • journal article
    • research article
    • Vol. 19  (2) , 111-114
Abstract
Dispersed tumor cells from primary human renal adenocarcinomas showed typical clonogenic growth in soft agar in 7 of 31 instances. In vitro chemotherapy sensitivity testing was performed successfully for 5 of the 7 clonogenic tumors. Extensive inherent resistance to the cytotoxic effects of many standard and experimental chemotherapeutic agents was observed. Occasionally, a single drug showed marked cytotoxicity. Similar results were obtained with soft agar clonogenic chemotherapeutic drug sensitivity assays performed on cells from human renal carcinoma xenografts. These in vitro results agree with clinical experience in the chemotherapy of advanced renal carcinoma. It remains to be determined if the sensitivity or resistance to chemotherapeutic drugs of human renal carcinomas noted in vitro reflects the characteristics of the tumors in vivo. The soft agar assay can be evaluated as a method for selecting drugs for individual patients with renal carcinoma, a disease for which no chemotherapeutic treatment can be recommended at present.

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