Kluyveromyces lactis maintains Saccharomyces cerevisiae intron-encoded splicing signals.
Open Access
- 1 May 1989
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 9 (5) , 2208-2213
- https://doi.org/10.1128/mcb.9.5.2208
Abstract
The actin (ACT) gene from the budding yeast Kluyveromyces lactis was cloned, and the nucleotide sequence was determined. The gene had a single intron 778 nucleotides in length which possessed the highly conserved splicing signals found in Saccharomyces cerevisiae introns. We demonstrated splicing of heterologous ACT transcripts in both K. lactis and S. cerevisiae.This publication has 47 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Saccharomyces cerevisiae Has a U1-Like Small Nuclear RNA with Unexpected PropertiesScience, 1987
- S. cerevisiae U1 RNA is large and has limited primary sequence homology to metazoan U1 snRNACell, 1987
- An essential yeast snRNA with a U5-like domain is required for splicing in vivoCell, 1987
- A Subset of Yeast snRNA's Contains Functional Binding Sites for the Highly Conserved Sm AntigenScience, 1987
- Mutations in a yeast intron demonstrate the importance of specific conserved nucleotides for the two stages of nuclear mRNA splicingCell, 1986
- Molecular consequences of specific intron mutations on yeast mRNA splicing in vivo and in vitroCell, 1985
- A point mutation in the conserved hexanucleotide at a yeast 5′ splice junction uncouples recognition, cleavage, and ligationCell, 1985
- Lariat structures are in vivo intermediates in yeast pre-mRNA splicingCell, 1984
- Organization and Expression of Eucaryotic Split Genes Coding for ProteinsAnnual Review of Biochemistry, 1981