A High-Pressure Liquid Chromatographic Method for Analysis of Carbohydrates and Polyols from Lichens

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Abstract
An analysis of 80% ethanol-soluble carbohydrates and polyols (polyhydric alcohols) from the lichens Ramalina stenospora Mull. Arg. and R. complanata (Sw.) Ach., was made by a high-pressure liquid chromatographic method. Two chromatographic columns, .mu.Bondapak carbohydrate and Aminex Q-150S, were used. Of the 2 columns, the .mu.Bondapak column resolved the larger number of carbohydrates from synthetic mixtures; however, the Aminex Q-150S column was required for resolution of mannitol-glucose-sorbitol and arabitol-ribitol complexes. Aminex Q-150S was more efficient in the separation of polyols than monosaccharides. Utilization of high-pressure liquid chromatography was a rapid, sensitive method for analysis of carbohydrates and polyols routinely found in lichen extracts. It has the desirable features of small sample size, recoverable fractions, rapidity and ease of quantification of lichen metabolites.

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