Improved collection of mobilized CD34+ hematopoietic progenitor cells by a novel automated leukapheresis system

Publisher Website
Google Scholar
Abstract
BACKGROUND: For simplification of blood cell transplantation, an automated apheresis system that exploits a dual‐stage channel device for mononuclear cell (MNC) collection (Au‐toPBSC, designed for the COBE Spectra) was studied. STUDY DESIGN AND METHODS: The automated default software (AutoPBSC‐Default) and three software modifications of the harvest frequency during leukapheresis, referred to as Au‐toPBSC‐1.25, AutoPBSC‐1.75, and AutoPBSC‐2.75, were evaluated in comparison with the semiautomated Version 4.7 (V4.7) apheresis system in 119 leukapheresis procedures performed in 90 cancer patients treated with chemotherapy plus granulocyte–colony‐stimulating factor. CD34+ cell and platelet collection efficiency (CE); volume and cell composition of the leukapheresis components; and patient platelet and red cell (RBC) loss during leukapheresis were measured. RESULTS: The majority of collection measures evaluated with the AutoPBSC compared favorably to those obtained with the V4.7. CD34+ cell CE increased from 55 percent with V4.7 to 68 percent with the AutoPBSC‐Default (p = 0.05). The AutoPBSC provided lower platelet contamination in the collected component (1.18 × 1011 vs. 2.26 × 10′′ with the V4.7;p< 0.001). The volume of the AutoPBSC‐Default component was significantly lower (67 vs. 180 mL with the V4.7; p11 vs. 1.04 × 1011 with the V4.7; p<0.001). Modifications of the AutoPBSC to increase the harvest frequency by 1.25‐, 1.75‐, and 2.75‐fold resulted in increased CD34+ cell CE (77%, 75%, and 83%, respectively; p<0.001 in all cases), but also in reduced numbers of circulating platelets, higher platelet contamination of the component, and lower MNC purity than were seen with the AutoPBSC‐Default. CONCLUSION: The AutoPBSC offers the following advantages over the V4.7 system: a) better CE of CD34+ cells; b) reduced collection of platelets; c) reduced contamination of the leukapheresis component with granulocytes, platelets, and RBCs; d) reduced component volume; and e) automation.

This publication has 27 references indexed in Scilit: