Acute Toxicity to Juvenile Rainbow Trout (Salmo gairdneri) of Naturally Occurring Insect Juvenile Hormone Analogues

Abstract
Median lethal concentrations (LC50) of four insect juvenile hormone analogues (IJHA) for juvenile rainbow trout (Salmo gairdneri) were measured in bioassays with 4-h solution renewal. The most toxic compound tested was Δ4′-dehydrojuvabione, with a 48-h LC50 of 1.4 mg/liter and an estimated 96-h LC50 of approximately 0.8 mg/liter. Juvabione, epimeric mixtures of dihydrojuvabiones, and epimeric mixtures of juvabiols had 96-h LC50’s of 1.5, 1.8, and 2.0 mg/liter, respectively. All fish, including those that survived for 96 h, became lethargic soon after exposure to solutions of the IJHA, and their skins darkened. The toxicants were unstable in aqueous solution.

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