High-performance liquid chromatographic determination of p-hydroxybenzoate esters in cosmetic lotions and creams.

1 January 1986

journal article
research article
Published by Pharmaceutical Society of Japan in Eisei kagaku

Vol. 32 (1) , 34-38
https://doi.org/10.1248/jhs1956.32.34

Abstract

A method for quantitative analysis of p-hydroxybenzoate esters (parabens) in cosmetic lotions and creams was developed by high-performance liquid chromatography (HPLC). Parabens were separated from the cosmetic base and other ingredients by bi-layer (upper; celite, lower; silica gel) column chromatography with Hexane-AcOEt (80:20). The eluate was extracted with acetonitrile. The acetonitrile layer was concentrated in vacuo and determined by HPLC on Develosil ODS by using a mobile phase of methanol-water (65:35, v/v). Recoveries of parabens obtained from model cosmetics were more than 95%. This method with clean-up procedure on bi-layer column chromatography and liquid extraction was much suitable for the determination of parabens in cosmetic lotions and creams.

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