The variations in the antigenic composition of Mycobacterium tuberculosis during the growth cycle as measured by the passive hemagglutination and precipitation reactions

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Abstract
The erythrocyte-sensitizing ability (ESA), as measured by the bis-diazotized-benzidine hemagglutination test, was determined for the extractable and culture filtrate antigens of three strains of mycobacteria (H37Rv, H37Ra, and BCG). The ESA of the precipitated and supernatant fractions obtained from these preparations by precipitation with ammonium sulfate, was found to vary according to the age of the cultures: the concentration of antigens decreased in the bacillary extracts and increased in the corresponding culture filtrates with the aging of the mycobacterial cultures.The variation in the antigenic composition of bacterial extracts and of culture filtrates during the growth cycle was also demonstrated by immunodiffusion techniques in agar gel. However, all extractable antigens of a given Mycobacterium did not appear in the culture filtrates.

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