GENETICAL MUTANTS INDUCED BY ETHYL METHANESULFONATE INSACCHAROMYCES

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Abstract
Ethyl methanesulfonate (EMS) has been shown to be an effective mutagen in Saccharomyces. In one experiment, 844 mutants were discovered by testing 2.28 x 104 survivors after treatment with 3% EMS. The mutants obtained included a large variety of auxotrophs, many of which required multiple growth factors. Some interesting mutants were obtained which could not grow on complete synthetic media, or which were inhibited. Certain mutants had lost mating type specificity. The unusually high frequency of multiple requirement, auxotrophic mutants, leaky mutants, poor germination and revertants, together with data obtained from genetical analysis of these EMS-induced mutants suggest that EMS is capable of inducing V-type position effects in Saccharomyces. This view will be dealt with in detail in a subsequent paper.