Histologie Investigation Of Glycol Methacrylate Embedded Chick Embryonic Tissue

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Abstract
Specific light microscopic investigations (i.e., histochemical) of early embryonic material are hindered by difficulties in processing and obtaining tissue sections of good quality. The advent of glycol methacrylate (GMA) as an embedding medium now provides a means to overcome these inherent problems with this tissue. Investigations were carried out to assess the histological results produced by different fixatives and times of fixation of GMA embedded 5 day chick embryonic tissue. Optimum cellular preservation of all tissues occurred following fixation in a mixture of acetic acid, 95% ethanol and neutral buffered formalin (AAF). These procedures can be used for more comprehensive examination of all types of early embryonic material.