Specificity of Factor Xa in the cleavage of fusion proteins
- 1 February 1993
- journal article
- Published by Springer Nature in Protein Journal
- Vol. 12 (1) , 1-5
- https://doi.org/10.1007/bf01024906
Abstract
The precursor protein honey bee prepromelittin has been expressed as a fusion protein inEscherichia coli joined to the C-terminus of a truncated form of the bacteriophage gene 10 protein via an engineered recognition sequence for Factor Xa. Factor Xa was found to cleave poorly at the engineered site, giving a low yield of the required prepromelittin. In contrast, cleavage on the C-terminal side of the sequence VLGR at residue 67 in the gene 10 sequence proceeded in high yield. Factor Xa may be inhibited by adjacent hydrophobic sequences on the C-terminal side of a potential cleavage site.Keywords
This publication has 11 references indexed in Scilit:
- The actions of melittin on membranesPublished by Elsevier ,2003
- Expression of honeybee prepromelittin as a fusion protein in Escherichia coliProtein Expression and Purification, 1991
- Factor Xa cleavage of fusion proteinsFEBS Letters, 1990
- [11] Secretion of heterologous proteins in Escherichia coliPublished by Elsevier ,1990
- [6] Use of T7 RNA polymerase to direct expression of cloned genesPublished by Elsevier ,1990
- Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.Journal of Biological Chemistry, 1987
- [29] Synthesis and sequence-specific proteolysis of hybrid proteins produced in Escherichia coliPublished by Elsevier ,1987
- Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elementsJournal of Molecular Biology, 1983
- A simple method for displaying the hydropathic character of a proteinJournal of Molecular Biology, 1982
- [28] Assay of coagulation proteases using peptide chromogenic and fluorogenic substratesPublished by Elsevier ,1981