The complete sequence of the 8·2 kb segment left of MAT on chromosome III reveals five ORFs, including a gene for a yeast ribokinase
- 1 November 1990
- Vol. 6 (6) , 521-534
- https://doi.org/10.1002/yea.320060609
Abstract
We report here the DNA sequence of a segment of chromosome III extending over 8·2 kb. The sequence was determined using the random clone strategy followed by oligonucleotide‐directed sequencing. The segments contains five long open reading frames, YCR521, 522, 523, 524 and 526, with only short distances between them. YCR523 (333 codons) endodes a ribokinase, a new function for yeast. YCR526 originates inside the MAT cassette, which is in continuity with the present segment, and extends over 358 codons outside of MAT. YCR524 (923 codons) codes for a putative membrane protein. YCR521, 522 and 524, have each been disrupted by insertion of a URA3 cassette and are non‐essential genes. An active ARS element is located within YCR523 or its vicinity.Keywords
This publication has 22 references indexed in Scilit:
- High Efficiency Transformation of Intact Yeast Cells by Electric Field PulsesNature Biotechnology, 1990
- Identification, purification, and cloning of a polypeptide (PRTF/GRM) that binds to mating-specific promoter elements in yeast.Genes & Development, 1990
- The codon adaptation index-a measure of directional synonymous codon usage bias, and its potential applicationsNucleic Acids Research, 1987
- GCN4 protein, synthesize in vitro, binds HIS3 regulatory sequences: Implications for general control of amino acid biosynthetic genes in yeastCell, 1985
- A repressor (MATα2 product) and its operator control expression of a set of cell type specific genes in yeastCell, 1985
- Specific DNA binding of GAL4, a positive regulatory protein of yeastCell, 1985
- Identification and comparison of two sequence elements that confer cell-type specific transcription in yeastNature, 1985
- [12] One-step gene disruption in yeastPublished by Elsevier ,1983
- DNA sequence required for efficient transcription termination in yeastCell, 1982
- The sequence of the DNAs coding for the mating-type loci of saccharomyces cerevisiaeCell, 1981