Differential lectin binding to cellular membranes in the epidermis of the newborn rat.

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Abstract
Three fluorescein-labeled lectins exhibited specificity for the surface of cells in different layers of the epidermis in the newborn rat. An isolectin from seeds of Bandeiraea simplicifolia with specificity for .alpha.-D-galactosyl end groups labeled the basal and lower spinous cells; a lectin from Ulex europaeus exhibiting specificity for .alpha.-L-fucosyl units outlines the surface of spinous cells and a 2nd lectin from B. simplicifolia, with specificity for N-acetyl-D-glucosamine, labels the cornified cells. Appropriate blocking experiments confirmed the specific nature of the binding.