Improved preparation for the diagnosis of mycologic diseases

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Abstract
The direct demonstration of fungi in human skin, nail, or hair scrapings is facilitated by dissolving the alkaline clearing agent, potassium hydroxide (20%), in a solvent of 609} water and 40% dimethyl sulfoxidc (DMSO). With this reagent, hyphae can often be seen as well in 1 minute without heating as in 20 minutes or more with the classical plain aqueous alkaline solution. The DMSO-containing reagent is not recommended if more than 20 minutes will elapse between preparation specimen and microscopic examination.

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