MOUSE RED-CELL ROSETTE FORMATION BY SUBPOPULATIONS OF HUMAN LYMPHOCYTES

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 27  (1) , 172-177
Abstract
In 6 separate experiments, 7.2% of purified peripheral blood lymphocytes from normal donors formed rosettes with mouse erythrocytes (E). Normal T [thymus-derived] and B [bone marrow-derived] lymphocytes were separated according to their membrane properties, by E-rosette formation or fractionation on anti F(ab'')2 column. The results obtained in both separation procedures used were in agreement. T lymphocytes never formed mouse red cell rosettes. But enrichment of suspensions in B lymphocytes resulted in an increased percentage of mouse red cell rosette-forming cells. Peroxidase-positive monocytes did not form such rosettes. If all the mouse red cell rosette-forming cells were of B cell nature, not all the B lymphocytes formed rosettes with mouse erythrocytes, and this marker could be characteristic for a subset of Ig[immunoglobulin]-bearing B lymphocytes.