Determination by systematic deletion of the amino acids essential for catalysis by ricin A chain.
- 1 June 1992
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 89 (11) , 4869-4873
- https://doi.org/10.1073/pnas.89.11.4869
Abstract
The A chain of ricin is a RNA N-glycosidase that inactivates ribosomes by depurination of a single adenosine in 28S rRNA. Of the 267 amino acids in the protein, 222 (83%) could be deleted from one or another of 74 mutants without loss of the capacity of the protein to recognize a single nucleotide from among the 7000 in ribosomes or to catalyze hydrolysis.Keywords
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