Purification and characterization of a lipocortin‐like 33 kDa protein from guinea pig neutrophils
- 25 January 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 227 (2) , 131-135
- https://doi.org/10.1016/0014-5793(88)80883-4
Abstract
A lipocortin‐like, phospholipase A2 inhibitory 33 kDa protein was purified from guinea pig neutrophils. From amino acid composition and sequence data, this protein was found to have a high degree of homology to human lipocortin I. This protein inhibited porcine pancreatic phospholipase A2 activity in the presence of [3H]oleic acid‐labeled Escherichia coli membranes as substrate. Maximal inhibition amounted to 65% whereas 50% inhibition occurred at 83.5 nM. This protein showed F‐actin‐binding ability in a Ca2+‐dependent manner.Keywords
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