Endothelin‐induced intracellular Ca2+ mobilization through its specific receptors in murine peritoneal macrophages
- 11 March 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 280 (1) , 103-106
- https://doi.org/10.1016/0014-5793(91)80214-n
Abstract
We studied the presence of specific binding sites for endothelin (ET) and the effect of ET on cytosolic free Ca2+ concentration ([Ca2+]i) in murine thiogylcolate‐activated peritoneal macrophages. Scatchard analysis for binding experiments using [125I]ET‐1 or [125I]ET‐3 revealed the existence of a single class of binding sites. The binding parameters (K??? and B???) for [125I]ET‐1 were almost identical to those for [125I]ET‐3. In addition, unlabeled 3 ET isopeptides (ET‐1, ET‐2 and ET‐3) inhibited the specific binding of both ET‐1 and ET‐3 with similar inhibitory potencies. All 3 ET isopeptides caused an increase in [Ca2+]i in the same dose‐dependent manner (0.01–100nM). These results demonstrate the existence of an ET receptor with the same affinity for all isoforms that mediates the ET‐induced intracellular Ca2+ mobilization in murine peritoneal macrophages.Keywords
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