Studies on Spinach Chloroplast and Nuclear DNA Using Large-scale Tissue Preparations
- 1 March 1969
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 44 (3) , 377-384
- https://doi.org/10.1104/pp.44.3.377
Abstract
A method for the large-scale preparation of spinach chloroplasts using the Spinco Model L-4 zonal ultracentrifuge and for the extraction of DNA from the chloroplasts is described. Thirty-five percent of the chloroplast DNA (rho = 1.706 g/cc) differs from nuclear DNA (rho = 1.695 g/cc) in buoyant density. T(m), base composition, and renaturation properties. Sixty-five percent of the chloroplast DNA (rho = 1.696 g/cc) has the same buoyant density and T(m) as nuclear DNA, but it differs in base composition and renaturation properties.Keywords
This publication has 13 references indexed in Scilit:
- Repeated Sequences in DNAScience, 1968
- Buoyant density of tobacco and spinach chloroplast DNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1968
- Electron microscopy of DNA conformations in spinach chloroplastsJournal of Molecular Biology, 1968
- The satellite DNA's of some higher plantsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967
- In Vitro Protein Synthesis by Plastids of Phaseolus vulgaris. I. Localization of Activity in the Chloroplasts of a Chloroplast Containing Fraction from Developing LeavesPlant Physiology, 1967
- The DNA of Chloroplasts, Mitochondria, and CentriolesProgress in Nucleic Acid Research and Molecular Biology, 1967
- Chloroplast DNA from Tobacco LeavesScience, 1966
- [DEMONSTRATION BY CYTOCHEMICAL TECHNICS AND ELECTRON MICROSCOPY OF DESOXYRIBONUCLEIC ACID IN MITOCHONDRIA AND PROTOPLASTS OF ALLIUM CEPA].1965
- Deoxyribonucleic acid from the chloroplasts of Euglena gracilisBiochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects, 1964
- The deoxyribonucleic acid content of purified spinach chloroplastsArchives of Biochemistry and Biophysics, 1964