Paths of Phosphate Transfer in Normal Chick Embryo Cells and in Cells Infected with Vesicular Stomatitis Virus

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Abstract
The paths of phosphate transfer were compared during one-step virus growth. Phosphate entered the normal cell via an inorganic pool in reversible equilibrium with external phosphate, and passed to large-molecule phosphate from this pool or from organic acid-soluble phosphate. During the latent and exponential release periods of virus infection there was no detectable slowing or stimulation of the rate of gain of p32 until uptake ceased in nearly all fractions about half-way through exponential release. Negligible P or p32 entered or left deoxyribonucleic acid in normal or infected cells. Before and during exponential release there was no detectable lysis of nuclei, mitochondria or microsomes, and no loss of P32 was detected except for a late decrease in that of the sucrose-soluble ribonucleic acid of disrupted cells. This could be a secondary effect rather than an essential stage of virus growth. Gross lysis was evident in all fractions 20 hours after infection, with the exception of the acid-soluble inorganic fraction not in reversible equilibrium with the medium.