Cellular differentiation and development of pyloric mucosal metaplasia in the human gall‐bladder

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Abstract
The development of pyloric mucosal metaplasia (PMM) with regard to cellular differentiation in the human gallbladder was studied by mucln staining (paradoxical concanavalin A (Con A), galactose oxidase‐Schiff (GOS) and alcian blue (pH 2.5) PAS (AB‐PAS) and immunohistochemistry (pepsino‐gen II (Pgll) and SH‐9, and proliferating cell nuclear antigen (PCNA). PMM was divided into three stages of development by three‐dimensional (3D) computer graphic reconstruction analysis. In the early stage, a transitional zone of PCNA positive cells was observed between areas of SH‐9 and/or GOS reactive cells and class III and/or Pg II positive cells in flat monolayered epithelium. In the middle stage, shallow pits became apparent as areas enlarged, with these becoming deeper in the advanced stage, whereby SH‐9 and/or GOS reactive cells and class III and/or Pg II positive cells were observed at the upper and lower portion of the pits, respectively, with PCNA‐positive cells forming a narrow zone between the two cell populations. Consequently, the structure of PMM gradually resembles that of the normal gastric pyloric mucosa.

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