A Slime-Producing Microorganism in Dental Plaque of Rats, Selected by Glucose Feeding

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Abstract
The administration of a glucose-rich diet to a group of O-M rats was followed by development of massive amounts of plaque. The predominating microorganism isolated from these plaques was a filamentous form identified as Actinomyces viscosus. A. viscosus, growing in dialyzed actinomyces broth (BBL) containing excess of glucose, produced a viscous culture from which extracellular slime was isolated by precipitation with ethanol. Fractionation of the extracellular slime on DEAE-Sephadex revealed the presence of two major components, one of which contained mainly glucose, while the other was more complex and contained glucosamine, hexose and protein. Antibodies could be produced against extracellular antigens which were found to be present in the slime but only accounted for a minor portion of the material.

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