Crystalline insulin
- 1 May 1935
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 29 (5) , 1048-1054
- https://doi.org/10.1042/bj0291048
Abstract
Crystalline insulin separated from phosphate buffer in presence of ZnCl2 and containing 0.7% ash was converted by electrodialysis and precipitation from dilute HCl soln. with acetone into insulin hydrochloride having 0.02% ash. Samples of this material were crystallized from ammonium acetate buffer in presence of (a) ZnCl2, (b) CdCl2, (c) CoCl2, yielding products having (a) Zn 0.52%, (b) Cd 0.77%, (c) Co 0.44%. The fact that these metals occur in crystalline insulin in ca. the proportions of their atomic wts. indicates that they are probably chemically combined.This publication has 1 reference indexed in Scilit:
- A reversible inactivation of insulinBiochemical Journal, 1929