Speciation of Chromium by Ion-Pair Chromatography with Postcolumn Spectrophotometric Detection

Abstract
High performance ion-pair chromatographic method of Cr(III) and Cr(VI) separation using phosphate buffer containing tetrabutylammonium bromide as eluent was developed. Post column derivatization based on Cr(VI) reaction with diphenylcarbazide and spectrophotometry monitoring of eluate at 560 nm was employed with Cr(III) oxidation to Cr(VI) using cerium(IV) sulphate. Detection limit was estimated as 7 ng/ml Cr(IIl) and 13 ng/ml Cr(VI).

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