Effect of Sulfite and Ascorbic Acid on Mushroom Phenol Oxidase

Abstract
SUMMARY: In model systems containing mushroom phenol oxidase and o‐diphenolic or monophenolic substrates in phosphate buffer, pH 6.5, sulfite initially did not protect the ascorbic acid from oxidation by the quinones formed enzymatically. The sulfite, however, gradually decreased both the diphenolase and monophenolase activity of the enzyme and thereby diminished the overall destruction of ascorbic acid. Under certain conditions, ascorbic acid counteracted the sulfite inhibition of the monophenolase activity of the enzyme.

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