Identification of coagulase-positive Staphylococcus intermedius and Staphylococcus hyicus subsp. hyicus isolates from veterinary clinical specimens

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Abstract
Coagulase-positive isolates of S. intermedius from dogs and coagulase-positive isolates of S. hyicus ssp. hyicus from a pig and cows were identified initially by a simplified scheme which can be readily performed in a routine diagnostic laboratory. Characters tested in the scheme included coagulase activity, colony pigment and aerobic acid production from maltose. The identity of these isolates was confirmed by DNA-DNA hybridization experiments. The strains of S. hyicus ssp. hyicus isolated from porcine sources were positive for protein A; the strains recovered from bovine mastitic milk were negative for protein A.