Characterization of the sat4 gene encoding a streptothricin acetyltransferase in Campylobacter coli BE/G4
- 1 July 1994
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 120 (1-2) , 13-17
- https://doi.org/10.1016/0378-1097(94)00168-5
Abstract
The sat4 streptothricin resistance gene from Campylobacter coli BE/G4 was cloned into pUC18, and its nucleotide sequence was determined. Streptothricin acetyltransferase activity was detected in Escherichia coli cells containing recombinant plasmid pAT132 which carries the sat4 gene as an insert. The deduced amino acid sequence displayed 21-27% amino acid identity with streptothricin acetyltransferases from E. coli and streptothricin producers Streptomyces lavendulae and Streptomyces noursei. The sat4 gene was detected by hybridization in clinical and environmental isolates of Campylobacter spp.Keywords
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