Immunochemical characterization of casein from rabbit mammary gland
- 1 September 1978
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 173 (3) , 877-883
- https://doi.org/10.1042/bj1730877
Abstract
1. Excellent precipitating antibodies to rabbit recombined casein polypeptides were obtained in a sheep after 8 weeks of immunization with rabbit recombined polypeptides coupled to Sepharose-albumin. 2. The antiserum was assessed for specificity by several immunochemical techniques and was monospecific when tested against acid-precipitated casein, recombined casein and extracts of lactating rabbit mammary tissue. 3. A specific anti-casein immunoglobulin fraction was prepared by immunoadsorption of the antiserum by using Sepharose-recombined casein as immunoadsorbent. 4. The specific anti-casein immunoglobulin was used to prepare a Sepharose-anti-casein immunoadsorbent for the isolation of casein from extracts of rabbit mammary tissue.This publication has 14 references indexed in Scilit:
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