Transposon donor plasmids, based on ColIb-P9, for use in Pseudomonas putida and a variety of other Gram negative bacteria
- 1 June 1985
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 200 (1) , 65-67
- https://doi.org/10.1007/bf00383313
Abstract
The properties of pLG221, a derivative of the ColIb plasmid carrying the transposon Tn5 are described. This plasmid can be used to introduce Tn5 by conjugation from Escherichia coli into a variety of Gram negative bacteria outside the host range for maintenance of ColIb. Plasmid pLG221, and a similar plasmid pLG223 carrying Tn10 may be of general utility as vectors for transposon-mediated mutagenesis in a variety of Gram negative bacteria.This publication has 22 references indexed in Scilit:
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