A Study of Calf‐Thymus Histone H2B Using 13C Magnetic Resonance
- 1 November 1976
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 70 (2) , 403-408
- https://doi.org/10.1111/j.1432-1033.1976.tb11030.x
Abstract
Calf thymus histone H2B has been studied by means of 13C magnetic resonance. Most sidechain resonances have been assigned. Analysis of 13C peak intensities by a simple computer‐aided method proves that 13C magnetic resonance spectroscopy can he used to monitor aggregation phenomena more accurately than 1H magnetic resonance or other physico‐chemical methods.Re‐examination of 1H spectral simulations shows substantial agreement with 13C results. It is concluded that in 0.1 M added NaCI the H2B aggregates include the chain segment from 50 (± 5)to 108 (± 5).This publication has 12 references indexed in Scilit:
- Investigation of the conformational and self-aggregational processes of histones using hydrogen and carbon-13 nuclear magnetic resonanceBiochemistry, 1975
- Conformations and interactions of histone H2A (F2A2, ALK)Biochemistry, 1975
- Carbon-13 NNIR of the protected tetrapeptides TFA-Gly-Gly-L-X-L-Ala-OCH3, where X stands for the 20 common amino acidsJournal of Magnetic Resonance (1969), 1974
- The structure and properties of histone F2a comprising the heterologous group F2a1and F2a2studied by13C nuclear magnetic resonanceNucleic Acids Research, 1974
- CONFORMATIONS AND INTERACTIONS OF HISTONES AND THEIR ROLE IN CHROMOSOME STRUCTURE *Annals of the New York Academy of Sciences, 1973
- Simple Computer-Aided Approach for the Analyses of the Nuclear-Magnetic-Resonance Spectra of Histones. Fractions Fl, F2al, F2B, Cleaved Halves of F2B and F2B middotDNAEuropean Journal of Biochemistry, 1972
- An Investigation of the Conformational Changes of Histone F2b by High Resolution Nuclear Magnetic ResonanceEuropean Journal of Biochemistry, 1970
- An Investigation of the Conformational Changes of Histones F1 and F2a1 by Proton Magnetic Resonance SpectroscopyEuropean Journal of Biochemistry, 1970
- Carbon-13 magnetic resonance studies of carbon-13 enriched amino acidsBiochemical and Biophysical Research Communications, 1969
- Studies on histones. 7. Preparative methods for histone fractions from calf thymusBiochemical Journal, 1964