Factors affecting the chemiluminescent response of fish phagocytes

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Abstract
The effects of several factors on the phagocytic activity of cells isolated from the pronephros of striped bass, Morone saxatilis, were measured using a chemiluminescence (CL) assay. The CL responses of phagocytes to varying concentrations of bacteria, phorbol myristate acetate (PMA), and zymosan were shown to be dose‐dependent. Incubation of phagocytes with PMA resulted in a decrease in cell numbers related to the concentration of PMA used in the assay. Opsonization of Aeromonas hydrophila with normal pooled bass serum decreased the number of colony forming units present in suspension while enhancing the CL response by striped bass phagocytes. Opsonization of zymosan also resulted in an enhanced CL response. Aeromonas hydrophila and Aerococcus viridans killed by heat treatment, incubation with formalin, or exposure to UV radiation elicited little or no CL when incubated with phagocytes.