Biosynthesis of the thiazole moiety of thiamin (vitamin B1) in higher plant chloroplasts.
- 15 March 1991
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 88 (6) , 2042-2045
- https://doi.org/10.1073/pnas.88.6.2042
Abstract
The chloroplast stromal proteins from spinach condense the two heterocyclic moieties of vitamin B1--4-methyl-5-(beta-hydroxyethyl)thiazole and 2-methyl-4-amino-5-hydroxymethylpyrimidine--in the presence of MgATP. We have taken advantage of this observation to study thiazole synthesis. We present data indicating that pyruvate, glyceraldehyde 3-phosphate, tyrosine, cysteine, and MgATP are required for this synthesis and that pyruvate and glyceraldehyde 3-phosphate can be replaced by 1-deoxy-D-threo-2-pentulose. The thiazole synthesis occurs at a sustained, low rate of 140 pmol per mg of protein per hr.Keywords
This publication has 16 references indexed in Scilit:
- Precursor of carbon atom five and hydroxymethyl carbon atom of the pyrimidine moiety of thiamin in Escherichia coli.Journal of Nutritional Science and Vitaminology, 1983
- The origin of the carbon chain in the thiazole moiety of thiamine in Escherichia coli: Incorporation of deuterated 1-deoxy-D-threo-2-pentuloseBiochemical and Biophysical Research Communications, 1981
- [12] High-pressure liquid chromatography of thiamine, thiamine analogs, and their phosphate estersPublished by Elsevier ,1979
- [10] Fluorometric determination of thiamine and its mono-, di-, and triphosphate estersPublished by Elsevier ,1979
- The origin of the nitrogen atom in the thiazole ring of thiamine in Escherichia coliBiochimica et Biophysica Acta (BBA) - General Subjects, 1978
- Plant pyruvate dehydrogenase complex purification, characterization and regulation by metabolites and phosphorylationBiochimica et Biophysica Acta (BBA) - Enzymology, 1977
- Biosynthèse du thiazole de la thiamine chez Escherichia coliBiochimie, 1977
- The origin of the sulfur atom in thiamineBiochimica et Biophysica Acta (BBA) - General Subjects, 1977
- The biosynthesis of the thiazole moiety of thiamine in Salmonella typhimurium☆Biochimica et Biophysica Acta (BBA) - General Subjects, 1976
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976