Expression of different mitogen‐regulated protein/proliferin mRNAs in Ehrlich carcinoma cells

Abstract
Results from in vivo and from serum‐free primary cultures of Ehrlich cells suggest that the expression of mitogen‐regulated protein/proliferin (MRP/PLF) mRNAs is not essential for proliferation of this murine tumor. Two sizes for MRP/PRL‐related open reading frames (ORFs) have been detected by reverse transcription/PCR amplification. They are almost identical to that reported for PLF‐1; but 20% of the amplified cDNA included a shorter ORF, which lacks the entire sequence corresponding to that of the exon 3 of the mrp/plf genes. Ehrlich carcinoma may represent a good model to study regulation of expression and physiological roles of MRP/PLFs in vivo.