Tetrahedral Intermediate in a Specific α-Chymotrypsin Inhibitor Complex Detected by Laser Raman Spectroscopy
- 1 August 1975
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 189 (4200) , 384-386
- https://doi.org/10.1126/science.238285
Abstract
Laser Raman spectroscopy indicates that inhibition of alpha-chymotrypsin by 2-phenylethaneboronic acid occurs in two steps: (i) the formation of a loosely bound complex, in which the boron remains in a trigonal configuration; and (ii) reaction of the boron with a functional group in the catalytic site of the enzyme, resulting in a reversible, stable tetrahedral adduct.Keywords
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