R46-derived recombinant plasmids affecting DNA repair and mutation in E. coli
- 1 June 1982
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 186 (1) , 140-144
- https://doi.org/10.1007/bf00422926
Abstract
Recombinant plasmids which render their host less mutable and more sensitive to some DNA-damaging agents have been isolated from the N-group plasmid R46. These plasmids have been physically mapped and found to originate from the region of R46 that has been deleted in pKM101. This deleted region is well removed from the muc region of R46 and pKM101 which is responsible for the mutator effects of these plasmids. The effect of these anti-mutagenic plasmids on the ability of pKM101 to complement umuC mutations has been examined, and they have been found to inhibit the complementation of such strains. We propose that these plasmids may code for a negative control function acting on the muc gene.This publication has 28 references indexed in Scilit:
- Inducibility of a gene product required for UV and chemical mutagenesis in Escherichia coli.Proceedings of the National Academy of Sciences, 1981
- The plasmid cloning vector pBR325 contains a 482 base-pair-long inverted duplicationGene, 1981
- A physical and genetic map of the IncN plasmid R46Plasmid, 1981
- Distribution among incompatibility groups of plasmids that confer UV mutability and UV resistanceMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1980
- Extraction of nucleic acids from agarose gelsAnalytical Biochemistry, 1980
- Escherichia coli recA gene product inactivates phage lambda repressor.Proceedings of the National Academy of Sciences, 1978
- Isolation and characterization of mutants of Escherichia coli deficient in induction of mutations by ultraviolet lightMolecular Genetics and Genomics, 1977
- Plasmid (pKM101)-mediated enhancement of repair and mutagenesis: Dependence on chromosomal genes in Escherichia coli K-12Molecular Genetics and Genomics, 1977
- A simple method for the preparation of large quantities of pure plasmid DNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1975
- Resistance to the Bactericidal Effect of Ultraviolet Radiatic Conferred on Enterobacteria by the Colicine Factor collJournal of General Microbiology, 1965