Involvement of IL‐1 family proteins in p38 linked cellular senescence of mouse embryonic fibroblasts
- 28 August 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 575 (1-3) , 30-34
- https://doi.org/10.1016/j.febslet.2004.08.033
Abstract
Senescence of mammalian cultured cells is essentially organized by a machinery of cell division and cellular stresses induced by various extracellular stimuli. Here, we show that in mouse embryonic fibroblasts (MEFs) culture in vitro, expression of an inflammatory cytokine, interleukin-1beta (IL-1beta) and its antagonist, IL-1 receptor antagonist (IL-1Ra) are induced by senescence. The kinetics of IL-1beta-expression was similar to that of p38 activation during MEFs culture. We also found a distinguishable accelerated senescence in cell growth in IL-1Ra deficient MEFs culture. Our results suggest that IL-1beta signaling pathway is involved in activation of p38 linked cellular senescence.Keywords
This publication has 27 references indexed in Scilit:
- IL-1 Plays an Important Role in Lipid Metabolism by Regulating Insulin Levels under Physiological ConditionsThe Journal of Experimental Medicine, 2003
- From cells to organisms: can we learn about aging from cells in culture?Experimental Gerontology, 2001
- The Role of Interleukin-1 in Interactive Senescence and Age-Related Human Endometrial CancerExperimental Cell Research, 1999
- Elevated Expression of Mitochondrial Cytochrome b and NADH Dehydrogenase Subunit 4/4L Genes in Senescent Human CellsExperimental Cell Research, 1995
- Pro-inflammatory Cytokines and Environmental Stress Cause p38 Mitogen-activated Protein Kinase Activation by Dual Phosphorylation on Tyrosine and ThreonineJournal of Biological Chemistry, 1995
- Fluorescent differential display: Arbitrarily primed RT‐PCR fingerprinting on an automated DNA sequencerFEBS Letters, 1994
- Differential extracellular matrix gene expression by fibroblasts during their proliferative life span in vitro and at senescenceJournal of Cellular Physiology, 1992
- Extension of the Life-Span of Human Endothelial Cells by an Interleukin-1αAntisense OligomerScience, 1990
- Biological properties of recombinant human monocyte-derived interleukin 1 receptor antagonist.Journal of Clinical Investigation, 1990
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970