RADIOIMMUNOASSAY OF WZ POLYMORPHIC ANTIGENS OF NORMAL HUMAN COLON AND THEIR RELATIONSHIP WITH ABH ANTIGENIC DETERMINANTS

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 32  (2) , 131-137
Abstract
Heat resistant, high molecular weight glycoproteins of the 3 WZ phenotypes (W+Z+, W-Z+ and W-Z-) were extracted from normal human colonic mucosa and labeled with 125I. More than 80% of the radioactivity of the W+Z+ antigens labeled with I125 was precipitated by natural or immune anti-W antibodies. About 60% of the W-Z+ 125I-labeled antigens were precipitated by immune anti-Z antibodies. No specific precipitation could be detected with W-Z-125I-labeled antigens. All the precipitin reactions were quantitatively inhibited with the crude antigen extracts of the corresponding phenotypes. The W and Z antigens were only found in the intestinal mucosa and there was a 100-fold increase in the relative concentrations between the small bowel and the rectum. The ABH antigens were found in all the digestive secretions studied, except those of the rectum, and their relative concentrations progressively decreased by about 100 between the small intestine and the rectum. The decrease in ABH concentration seems to be correlated to a WZ increase in the same regions.