Dependency of Renal Blood Flow on Prostaglandin Synthesis in the Dog

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Abstract
Inhibition of prostaglandin synthesis in chloralose-anesthetized dogs reduced renal blood flow, and this reduction closely correlated (r=0.92, P 2 . We used solvent extraction and thin-layer chromatography coupled with parallel bioassay to identify and assay the PGE- and PGF-like substances (expressed as PGE 2 and PGF 2α equivalents). Either of two antiinflammatory acids, indomethacin or meclofenamate, that inhibited conversion of 14 C-arachidonic acid to prostaglandins in renal homogenates decreased the basal concentration of a PGE-like substance in renal venous blood to 0.06 ± 0.02 ng/ml from a mean control value of 0.34 ± 0.10 ng/ml ( P 2 participates in maintaining renal vascular tone which heretofore has been ascribed to autonomous, intrinsic renal arteriolar activity.