Studies on isolated smooth muscle cells. I. Continuous observation of contraction of single smooth muscle cells isolated from Vas deferens of guinea pig.

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Abstract
Simple technique for isolation and continuous observation of contractile response of single smooth muscle cells from vas deferens of guinea pig was examined for investigation of contractile mechanism of smooth muscle instead of use of whole tissue. Cells were isolated by digestion with collagenase followed by mechanical agitation. Intact cells which were not stained by trypan blue and spindle shape were capable of contraction by application of Ca in a medium containing higher concentration of K. For continuous observation of contracting cells, the cells were perfused continuously with incubation medium on silicon-coated slide glass at 37.degree. and degree of contraction was determined photographically with use of phase contrast microscope. By this procedure, many isolated cells were observed before and through contraction with the same preparation.

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