ETHACRYNIC-ACID AND PIRIPROST AS ENHANCERS OF CYTO-TOXICITY IN DRUG-RESISTANT AND SENSITIVE CELL-LINES
- 1 July 1988
- journal article
- research article
- Vol. 48 (13) , 3622-3625
Abstract
Ethacrynic acid and piripost (6,9-deepoxy-6,9-(phenylimino)-.DELTA.6,8-prostaglandin I1) have been shown to potentiate the cytotoxic activity of chlorambucil in rat and human tumor cell lines. Walker 256 rat breast carcinoma cells (WS), with acquired resistance to nitrogen mustards (WR), and two human colon carcinoma cell lines, HT 29 and BE, were sensitized to chlorambucil when either ethacrynic acid or piriprost was administered at the same time as the alkylating agent. Both as single agents and in combination with chlorambucil, there was inhibition of glutathione-S-transferase activity as measured with 1-chloro-2,4-dinitrobenzene as a substrate. A depletion in intracellular glutathione was also evident following ethacrynic acid alone or in combination with chlorambucil. Thus, diuretic plant phenols or prostaglandin analogues may have potential therapeutic utility in combination with alkylating agents.This publication has 18 references indexed in Scilit:
- Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridinePublished by Elsevier ,2004
- A proposed mechanism of resistance to cyclophosphamide and phosphoramide mustard in a Yoshida cell line in vitroCancer Chemotherapy and Pharmacology, 1986
- GLUTATHIONE CONJUGATES - IMMOBILIZED ENZYME-SYNTHESIS AND CHARACTERIZATION BY FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY1986
- Inhibition of purified rat liver glutathione S-transferase isozymes by diuretic drugsBiochemical Pharmacology, 1985
- INHIBITION OF RAT-LIVER GLUTATHIONE S-TRANSFERASES BY PIRIPROST - KINETICS OF THE INHIBITION AND PRELIMINARY EVIDENCE THAT PIRIPROST MAY BE A POOR ALTERNATIVE SUBSTRATE FOR THESE ENZYMES1985
- INCREASED GLUTATHIONE-S-TRANSFERASE ACTIVITY IN A CELL-LINE WITH ACQUIRED-RESISTANCE TO NITROGEN MUSTARDS1985
- Inhibition of soluble glutathione S-transferase by diuretic drugsBiochemical Pharmacology, 1984
- SELECTIVE CYTO-TOXICITY OF HALOETHYLNITROSOUREAS IN A CARCINOMA CELL-LINE RESISTANT TO BIFUNCTIONAL NITROGEN MUSTARDS1982
- Enhancement of anti-cancer activity of cytotoxic chemotherapy with protection of normal tissues by inhibition of P.G. synthesisBiochemical Pharmacology, 1978
- Inhibition of prostaglandin synthetase by anti-tumour agentsChemico-Biological Interactions, 1977