Involvement of Urokinase and its Receptor in the Invasiveness of Human Prostatic Carcinoma Cell Lines
- 1 August 1991
- journal article
- research article
- Published by Cognizant, LLC in Cancer Communications
- Vol. 3 (8) , 255-264
- https://doi.org/10.3727/095535491820873146
Abstract
We have investigated the role of urokinase (UK) and its cell-surface receptor in determining the invasiveness of prostate cancer cells. Three human cell lines, DU-145, PC-3 and LNCaP, that differ in androgen-responsiveness and growth characteristics, were tested. Analysis of the conditioned medium by an enzyme-linked immunosorbent assay showed secretion of UK by DU-145 (63 ng/mL/106 cells/48 hr) and PC-3 (682 ng/mL/106 cells/48 hr), but absence of secretion by LNCaP cells. Western blot analysis and enzyme activity assay of the conditioned medium confirmed these results. Scatchard analysis of radioligand binding with acid pretreated cells showed the presence of a single population of high affinity UK receptors on DU-145 cells (93,000 sites/cell, Kd = 0.9 nM) and PC-3 cells (25,000 sites/cell, Kd = 1.0 nM) but not on LNCaP cells. DU-145 and PC-3 cells were found to be highly invasive in in vitro invasion assays: 4.5 ± 0.5% and 6.5 ± 0.5%, respectively, of total tumor cells (∼ 2 × 105) had penetrated reconstituted basement membrane (Matrigel™) in a 72 hr incubation in serum-free growth medium. Under similar conditions, No Reference information available - sign in for access. No Citation information available - sign in for access. No Supplementary Data. No Article Media No MetricsKeywords
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