Arsenate as a potential negative selection agent for deficiency variants in cultured plant cells

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Abstract
Sodium arsenate is toxic to cultured soybean [Glycine max (L.) Merr.] cells, killing virtually 100% of the cells during a 24-h exposure at a 1–2 mM concentration. However, when growth is previously halted by nitrogen deprivation 50–100% of the cells survive arsenate treatment. Because of this growthdependent toxicity, arsenate has promise as a negative selection agent for cultured plant cells. Using arsenate (2 mM) I was able to select from among 2×107 cells a cell line with a growth requirement for an amino acid mixture. This trait was maintained through 9 months of passage but then was lost.