Development of a fluorimeter using laser-induced single-shot fluorescence lifetime spectroscopy

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Abstract
The developed laboratory prototype fluorimeter is the first step to a new in-situ instrument, and is based on a pulsed nitrogen laser (pumping a color dye laser and the laserbeam passing through a frequency doubler) with a pulse width less than 1 nsec. With such a short excitation pulse it is possible to measure the exponential decay of the fluorescence from the aromatic compounds and thus determine the fluorescence lifetime-curves, which are typically in the region of 5-40 nsec. The emitted fluorescence is collected simultaneously in 35 channels in the wavelength region 250-600 nm. If the fluorescence falls within the transmission areas of the interference filters in each channel the light will be collected by a plastic light guide (doped PMMA) in the actual channel and transmitted to the channels photo multiplier tube (PMT). (The use of the plastic light guide improves the sensitivity). The signal from the PMT is passed on to a 200 MHz 8-bit flash AID-converter connected to a local memory. From this local memory the digital lifetime curves from each channel are transmitted to a computer for presentation of the 3-dimensional spectrum. This spectrum has been obtained with a single laser shot.

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